A common question from newbies is "How can I reference the root path of my application from controls?". It is common for many server side controls to have properties such as src (source) and hyperlinks/URL etc. ASP.NET has a neat syntax that you can use to reference the root of the applications directory in any server-side controls URL property. This syntax will relief you of having to place relative pathis in your apps directory hierachy.
Simply prefix the path with the ~ (tilda) character. During the compilation process of the server-side control, ASP.NET will translate that ~ into Request.ApplicationPath.
An example would look like <img src="~/images/logo.gif" runat="server">. Keep in mind that this will work for SERVER-SIDE controls only!